PRESERVATION OF MICROBIAL CULTURES · Different techniques are used for maintenance and preservation of different organisms based on their properties. · Techniques for the Preservation of microbes broadly divided into two categories. I. Continuous metabolic active state preservation technique. II. Suspended metabolic rate preservation technique. I) CONTINUOUS METABOLIC ACTIVE STATE PRESERVATION TECHNIQUE In this technique, organisms preserved on nutrient medium by repeated sub-culturing. In this technique, any organisms are stored by using general nutrient medium. Here repeated sub-culturing is required due to depletion or drying of nutrient medium. This technique includes preservation by following methods. 1) Periodic Transfer · Organisms grown in general media on slant, incubated for particular period at particular temperature depending on the cha...
Bacteriological analysis of water – Most Probable Number (MPN) Technique AIM To check the potability of the given Drinking water sample by Most Probable Number (MPN) Technique. PRINCIPLE Washing receives human and animal waste from a primary source of water borne disease. Direct heating produces detecting and quantifying the full spectrum of pathogens and identifying their source are time consuming sources because of their variable accordance survival rate and origin. Hence, an indicator system has been identified as the best method for evaluating the microbiological quantify of water. Coliform especially faecal coliform test is considered as the most reliable test new available for detecting the presence of contamination of an intestinal origin. The c...