Differential Centrifugation Differential centrifugation is the most common separation method which was used to separate the particles according to size, shape and mass. Particles of different densities or sizes in a suspension will sediment at different rates, with the larger and denser particles sedimenting faster. It is an Ultracentrifugation method. The simplest form of separation by centrifugation is differential centrifugation, sometimes called Differential pelleting. Differential centrifugation is used for the separation of cellular organelles and macromolecules. Usually, Fixed angle rotors are used for Differential centrifugation. Density Gradient Centrifugation Density Gradient Centrifugation is the separation performed in a supporting column of solution in which the density and solution concentration increase toward the bottom of the centrifuge tube. It is an important technique used for the purification of Proteins and Nucleic acids. Two different types of density gradient c