SYNCHRONOUS GROWTH Synchronous or Synchronized culture is a microbiological culture or a cell culture that contains cells that are all in the same growth stage. Synchronous growth helps studying particular stages or the cell division cycle and their interrelations. A Synchronous culture can be obtained either by manipulating environmental conditions such as by repeatedly changing the temperature or by adding fresh nutrients to cultures as soon as they enter the Stationary phase, or by physical separation of cells by Centrifugation or Filtration. An excellent and most widely used method to obtain synchronous cultures is the Helmstetter-Cummings Technique in which an unsynchronized bacterial culture is filtered through Cellulose nitrate membrane filter. The loosely bound bacterial cells are washed from the filter, leaving some cells tightly associated with th...