REQUIRED CONCENTRATIONS AND TIMES FOR CHEMICAL DESTRUCTION OF MICROORGANISMS Disinfectants Microorganisms Disinfectant concentration Time Chlorine Mycobacterium tuberculosis 50 ppm 50 sec Entamoeba cyst 0.1 ppm 150 min Hepatitis A Virus 3 ppm 30 min Ethyl alcohol Staphylococcus aureus 70 % 10 min Escherichia coli 70 % 2 min Poliovirus 70 % 10 min Hydrogen peroxide Staphylococcus aureus 3 % 12.5 sec Neisseria gonorrhoea 3 % 0.3 sec Herpes simplex virus 3 % 12.8 sec Quaternary Ammonium compounds Staphylococcus aureus 450 ppm 10 min Salmonella typhi 300 ppm 10 min