Saran's Learn Microbiology

 

Binocular Light Microscope ·        Illuminator - This is the light source located below the specimen. ·        Condenser - Focuses the light through the specimen. ·        Stage - The platform that holds the specimen. ·        Stage Clips – Hold the slide in place. ·        Objective Lens - The lens that is directly above the stage. ·        Nose Piece - The portion of the body that holds the objectives over the stage. ·        Field Diaphragm­ - Controls the amount of light into the condenser. ·        Base - Bottom of the microscope. ·        Coarse Focusing Knob - Used to make relatively wide focusing adjustments to the microscope. ·        Fine Focusing Knob - Used to make relatively small adjustments to the microscope. ·        Body – The microscope body. ·        Ocular Lens (or) Eye Piece - Lens on the top of the body tube. It has a magnification of 10× normal vision. ·        Immersion oil is used to improve the resolution o

 

  MICROSCOPY ·                       Microscopy is the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye).  A microscope is an optical instrument that magnifies objects otherwise too small to be seen, producing an image in which the object appears larger.  A microscope uses a lens or a combination of lenses to produce highly magnified images of small specimens or objects especially when they are too small to be seen by the naked eye. A light source is used to make it easier to see the subject matter.   Most photographs of cells are taken using a microscope, and these pictures can also be called Micrographs.  Three parameters are especially important in microscopy: Magnification, Resolving power and Numerical aperture. i) Magnification ·        Magnification is a measure of how much larger a microscope (or set of lenses within a microscope) causes an object t