Saran's Learn Microbiology

  FLUORESCENCE MICROSCOPE ·        The Fluorescence microscope is a specially modified Compound microscope furnished with an Ultraviolet (UV) radiation source and Filters that protect the viewer’s eye from injury by these dangerous rays. ·        The Fluorescence microscope was invented in the early part of the 19 th century by August Köhler, Carl Reichert and Heinrich Lehmann. ·        The most commonly used Fluorescence microscopy is Epifluorescence microscopy, also called Incident light or Reflected light Fluorescence microscopy. ·        Fluorescence microscopes employ an Objective lens that also acts as a Condenser. The Objective lens acts not only as a magnifier for the light emitted by the fluorescing object, but also as a Condenser for the Incident light. ·        Fluorescence microscopy uses Fluorescence dyes to examine the Structural organization and Spatial distribution of samples. ·        Fluorescence microscopy is particularly used to study Samples that are C

 

 

  POLARIZING MICROSCOPE ·        A Polarizing microscope is a type of microscope that uses polarized light to view specimens. ·        Polarizing microscopes are conventional microscopes with additional features that permit observation under Polarized light. ·        Although the invention of the polarizing microscope is typically attributed to David Brewster around 1815, Brewster clearly acknowledges the priority of Henry Fox Talbot, who published his work in 1834. ·        Polarizing microscope is commonly used to observe minerals, crystals, and other transparent or semi-transparent materials. It is also used to analyze the structure and properties of the above given materials. Conversion of Unpolarized light to Polarized light ·        Light is an electromagnetic wave. Although light waves can vibrate in all directions, in general, they are described as vibrating in two directions at right angles to each other. ·        Any light which vibrates in more than one directi