Saran's Learn Microbiology

ONLINE COURSE CONTENTS CHAPTER NUMBER TOPICS WEEK – 1 1 History of Microscopy 2 Parts of Microscope 3 Difference between Simple and Compound Microscope 4 Difference between Light and Electron Microscope 5 Bright Field Microscope 6 Dark Field Microscope 7 Polarizing Microscope 8 Fluorescent Microscope 9 Confocal Microscope 10 Phase Contrast Microscope 11 Differential Interference Contrast (DIC) Microscope 12 Electron Microscope 13 Difference between SEM and TEM 14 Scanned Probe Microscopy – Scanning Tunnelling Microscopy 15 Scanned Probe Microscopy – Atomic Force Microscopy WEEK – 2 16 A

 COAGULASE TEST AIM         To differentiate Coagulase positive Staphylococcus aureus from Coagulase negative Staphylococcus sp. ( Staphylococcus epidermis and Staphylococcus saprophyticus ). PRINCIPLE             Coagulase is an enzyme that works in conjugation with normal plasma components to convert Fibrinogen to Finbrin and causes Clotting of Plasma. This enzyme is a good indicator of pathogenic potential of Staphylococcus aureus . As 97 % of the strains of Staphylococcus aureus have proven to be Coagulase positive, the Coagulase test is useful for differentiating Staphylococcus aureus from other Gram positive, Catalase negative cocci.         Staphylococcus aureus produces two types of Coagulases. First one is Bound Coagulase and the Second one is Free Coagulase. Slide coagulase test is done to detect Bound coagulase, whereas Tube coagulase test is done to detect Free coagulase. Both Slide and Tube coagulase tests utilize Rabbit plasma treated with anticoagulant to interrup