Saran's Learn Microbiology

ONLINE COURSE CONTENTS CHAPTER NUMBER TOPICS WEEK – 1 1 History of Microscopy 2 Parts of Microscope 3 Difference between Simple and Compound Microscope 4 Difference between Light and Electron Microscope 5 Bright Field Microscope 6 Dark Field Microscope 7 Polarizing Microscope 8 Fluorescent Microscope 9 Confocal Microscope 10 Phase Contrast Microscope 11 Differential Interference Contrast (DIC) Microscope 12 Electron Microscope 13 Difference between SEM and TEM 14 Scanned Probe Microscopy – Scanning Tunnelling Microscopy 15 Scanned Probe Microscopy – Atomic Force Microscopy WEEK – 2 16 ...

 COAGULASE TEST AIM         To differentiate Coagulase positive Staphylococcus aureus from Coagulase negative Staphylococcus sp. ( Staphylococcus epidermis and Staphylococcus saprophyticus ). PRINCIPLE             Coagulase is an enzyme that works in conjugation with normal plasma components to convert Fibrinogen to Finbrin and causes Clotting of Plasma. This enzyme is a good indicator of pathogenic potential of Staphylococcus aureus . As 97 % of the strains of Staphylococcus aureus have proven to be Coagulase positive, the Coagulase test is useful for differentiating Staphylococcus aureus from other Gram positive, Catalase negative cocci.         Staphylococcus aureus produces two types of Coagulases. First one is Bound Coagulase and the Second one is Free Coagulase. Slide coagulase test is done to detect Bound coagulase, whereas Tube coagulase test is done to detect Fre...

STREAK PLATE TECHNIQUE AIM         To isolate the pure strains of bacteria by Streak plate technique (Quadrant streaking method). PRINCIPLE              The Streak plate technique is a rapid qualitative isolation method used for the isolation of a pure culture of the bacteria from the mixed population.  Streaking method was first developed by Loeffler and Gaffky in Robert Koch’s laboratory, which involves the dilution of bacteria by systematically streaking them over the exterior of the agar in a Petridish to obtain isolated colonies which will then grow into the number of cells or isolated colonies. The inoculum is streaked over the agar surface in such a way that it “thins out” the bacteria. Some individual bacterial cells are separated and well-spaced from each other. Aseptic techniques are used to maintain microbiological cultures and to prevent contamination of the...