DIFFERENCE BETWEEN BRIGHT FIELD MICROSCOPE AND DARK FIELD MICROSCOPE

DIFFERENCE BETWEEN BRIGHT FIELD MICROSCOPE AND DARK FIELD MICROSCOPE

BRIGHT FIELD MICROSCOPE	DARK FIELD MICROSCOPE
Formed a Dark image against Bright field.	Formed a bright image against Dark background.
Stained, fixed, and live specimens are observed.	We can see the living and unstained cells.
Specimen colour appears depending on the Stain colour.	Specimen appears as Bright.
Specimen background appears bright.	Specimen background appears dark.
Opaque disc is absent.	Opaque disc is present.
Specimen preparation or staining is a complex and lengthy process. Takes too much time.	No need to stain.
Shows the morphological as well as the internal structure of the specimen.	Shows the External structure of specimen in great detail.
Less expensive than Dark field microscope.	More expensive than Bright field microscope.
Easy to use.	Operating procedure is not easy as compared to bright field microscope.
Does not allow us to observe the Metal and Minerals.	Used to study for minerals and crystals, thin polymers, and some ceramics.

Comments

15 Days International Online Certificate Course on “FUNDAMENTALS OF MICROBIAL INSTRUMENTATION”

DAY CHAPTER NUMBER TOPICS Day – 1 21.01.2026 1 Orientation to the Microbiology Laboratory 2 Parts of Microscope 3 Bright Field Microscope and Dark Field Microscope 4 Difference between Simple Microscope and Compound Microscope Day – 2 22.01.2026 5 Electron Microscope 6 Difference between Light Microscope and Electron Microscope 7 Difference between Scanning Electron Microscope and Transmission Electron Microscope Day – 3 23.01.2026 Quiz 1 from Day 1 Lecture Videos and Day 2 Lecture Videos (20 Multiple Choice Questions) Day – 4 24.01.2026 8 An Introduction to Spectrophotometer 9 Preventive Measures in Spectrophotometer 10 Spectrophotometer - Applications, Adva...

International Four Weeks Online Certificate Course on “MICROBIOLOGY LABORATORY TECHNIQUES” (Phase - III)

ONLINE COURSE CONTENTS DAY CHAPTER NUMBER TOPICS Week - 1 1 Orientation to Microbiology Laboratory 2 Sterilization – Physical method (Drying, Dry heat and Moist heat) 3 Sterilization – Physical method (Filtration and Radiation) 4 Sterilization – Chemical method 5 Evaluation of Disinfectants 6 Parts of Microscope 7 Difference between Simple microscope & Compound microscope 8 Difference between Light microscope & Electron microscope 9 Difference between SEM and TEM 10 Culture medium 11 Guidelines for the Collection of Clinical Specimens 12 Collection of Clinical specimens 13 Transport of Clinical specimens Week – 2 14 ...

CONFLICT OVER SPONTANEOUS GENERATION (Spontaneous Generation, Francesco Redi, John Needham, Lazaro Spallanzani and Louis Pasteur's Swan Neck Flask Experiment)

SPONTANEOUS GENERATION · “Living organisms can arise from non-living things” is referred as Spontaneous generation (or) Abiogenesis. · For example, ü Mice from Grains ü Beetles from Dust ü Worms and Frogs from Mud ü Maggots from Rotting meat. · Aristotle (384 – 322 BC) (Greek Philosopher) (Father of Biology) proposed Spontaneous Generation Theory at 350 BC. · The microbiology starts when the disprove of Spontaneous Generation theory. FRANCESCO REDI (1626 – 1697) · Francesco Redi (Father of Parasitology) proposed “Theory of Biogenesis” – Life comes from Life in 1665. · Francesco Redi was the first who disproved the “Theory of Spontaneous Generation” by showing that ...

Saran's Learn Microbiology

Search This Blog