LOUIS PASTEUR
· Louis Pasteur
was the French Chemist, Biologist & Microbiologist, and “Father of Modern
Microbiology”.
·
Louis Pasteur
is a Chemistry Professor from France who disproved the Spontaneous Generation
Theory completely by his “Swan Neck Flask Experiment”.
·
Main contributions of Louis Pasteur includes
ü Disproved the Spontaneous
Generation Theory.
ü
First coined the term “Microbiology” for the study of organisms of microscopic
size.
ü
Louis Pasteur proposed the “Germ Theory of Disease”. He stated that
disease cannot be caused by bad air or vapor but it is produced by the
microorganisms present in air.
ü
Liquid media concept – Louis Pasteur was the first who used Nutrient
broth to grow microorganisms.
ü
Demonstrated the term Virulence (ability of microbe to cause disease) of
bacteria.
ü
Introduced the term “Attenuation” that means reduction of Virulence of
pathogens by prolonged cultivation.
ü
Discovered the Yeast fermentation in fruits (Conversion of sugar to
ethanol and CO2) fruit, Butyric acid fermentation and Lactic acid
fermentation (sugars could be converted to Lactic acid by bacteria).
ü
Sorted different microbes giving different taste of wine.
ü
Louis Pasteur selected a particular strain (Yeast – Saccharomyces
cerevisiae) for high quality wine.
ü
Louis Pasteur introduced the sterilization techniques and developed Steam
sterilizer, Hot air oven and Autoclave (developed by Charles Chamberland who
was the student of Louis Pasteur).
ü
Louis Pasteur described the method of Pasteurization of Milk and Wine.
ü
Louis Pasteur discovered that the protozoa Nosema bombycis is
responsible for causing Pebrine disease in silk worm. He suggested that disease
free caterpillars can eliminate the disease.
ü
The term “Vaccine” was first coined by Louis Pasteur.
ü
Louis Pasteur developed Rabies vaccine (a killed or inactivated microbe
to induce the immunity) against Rabies from the brains and spinal cord of
rabbit. On July 6, 1885, Louis Pasteur vaccinated Joseph Meister, a
nine-year-old boy who had been bitten by a rabid dog.
ü
Louis Pasteur also developed the Chicken cholera vaccine (Pasteurella multocida) (Attenuated
Vaccine) & Anthrax vaccine (Bacillus
anthracis) (Attenuated Vaccine).
ü
Pasteur Effect – Louis Pasteur unexpectedly discovery that the
Fermentation process could be arrested by passing air (oxygen) through the
fermenting fluid, a process known today as the “Pasteur effect”. He concluded
that this was due to the presence of a life-form that could function only in
the absence of oxygen. This led to his introduction of the terms “Aerobic” and “Anaerobic: to
designate organisms that live in the presence or absence of oxygen,
respectively.
ü
Louis Pasteur further proposed that the phenomena occurring during "Putrefaction" (decomposition of organic
matters) were due to specific germs that function under Anaerobic
conditions.
ü Molecular asymmetry - In studying crystals of Sodium ammonium tartrate, Louis Pasteur found that although they had the same chemical composition, they did not necessarily have the same structure. He noted that the molecules occurred in two mirror-image arrangements that could not be superimposed. This Molecular asymmetry, or Chirality (mirror image), is the foundation of a branch of science known as Stereochemistry.
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