Skip to main content

Zonal Centrifugation and Isopycnic Centrifugation

 Difference between Zonal Centrifugation and Isopycnic Centrifugation


Rate Zonal Centrifugation

Isopycnic Centrifugation

Low speed.

High speed.

Swing Rotor was used.

Fixed Angle or Swing Rotor was used.

Not completely sedimented.

Completely sedimented.

Short running time (Short duration).

Long running time (Long duration).

Gradient type is Continuous.

Gradient type is Continuous or Discontinious.

Separation was based on Size of molecules.

Separation was based on Density of molecules.

Particles are separated on the basis of Sedimentation rate.

Particles are separated on the basis of Sedimentation equilibrium.

Similar density but different molecular weight.

Similar molecular weight but different density.

Used for the separation of Proteins,  Enzymes, Hormones, Nucleic acids and Cell organelles.

Used for the separation of sub-cellular particles like Mitochondria, Lysosomes and Peroxisomes.

Comments

Popular posts from this blog

Four Weeks International Online Certificate Course on “BIOANALYTICAL TECHNIQUES (Part II) – Electrophoresis, Spectroscopy and Microbiology Lab Instruments (Phase - I)”

Four Weeks International Online Certificate Course on “BIOANALYTICAL TECHNIQUES (Part II) – Electrophoresis, Spectroscopy and Microbiology Lab Instruments (Phase - I)” ONLINE COURSE CONTENTS CHAPTER NUMBER TOPICS WEEK – 1 1 An Introduction to Electrophoresis 2 Forms and Types of Electrophoresis 3 Electrophoresis – Applications, Advantages and Limitations 4 Gel Electrophoresis 5 Agarose Gel Electrophoresis 6 Pulsed-field Gel Electrophoresis (PFGE) 7 SDS – PAGE 8 2-Dimensional (2D) Electrophoresis 9 Capillary Electrophoresis (CE) 10 Immunoelectrophoresis 11 Affinity Electrophoresis 12 Paper Electrophoresis 13 Cellulose Acetate Electrophoresis 14 ...

Four Weeks International Online Certificate Course on “BIOANALYTICAL TECHNIQUES (Part I) – Microscopy, Chromatography and Centrifugation (Phase - II)”

ONLINE COURSE CONTENTS CHAPTER NUMBER TOPICS WEEK – 1 1 History of Microscopy 2 Parts of Microscope 3 Difference between Simple and Compound Microscope 4 Difference between Light and Electron Microscope 5 Bright Field Microscope 6 Dark Field Microscope 7 Polarizing Microscope 8 Fluorescent Microscope 9 Confocal Microscope 10 Phase Contrast Microscope 11 Differential Interference Contrast (DIC) Microscope 12 Electron Microscope 13 Difference between SEM and TEM 14 Scanned Probe Microscopy – Scanning Tunnelling Microscopy 15 Scanned Probe Microscopy – Atomic Force Microscopy WEEK – 2 16 A...

International Online Short Term Certificate Course on “CHROMATOGRAPHY – AN OVERVIEW” (Phase - I) (for Faculties, Research Scholars and Students)

DAY CHAPTER NUMBER TOPICS Day – 1 04.11.2024 1 An Introduction to Chromatography 2 History of Chromatography 3 Theories of Chromatography 4 Chromatography – Applications, Advantages and Limitations Day – 2 05.11.2024 5 Thin Layer Chromatography (TLC) 6 High Performance Thin Layer Chromatography (HPTLC) 7 Difference Between TLC and HPTLC Day – 3 06.11.2024 8 Paper Chromatography 9 Difference Between Thin Layer Chromatography (TLC) and Paper Chromatography Day – 4 07.11.2024 10 Column Chromatography 11 Flash Column Chromatography Day – 5 08.11.2024 12 High Performance Liquid Chromatography (HPLC) 13 Dif...