International Four Weeks Online Certificate Course on “MICROBIOLOGY LABORATORY TECHNIQUES” (Phase - III)

ONLINE COURSE CONTENTS

DAY	CHAPTER NUMBER	TOPICS
Week - 1	1	Orientation to Microbiology Laboratory
	2	Sterilization – Physical method (Drying, Dry heat and Moist heat)
	3	Sterilization – Physical method (Filtration and Radiation)
	4	Sterilization – Chemical method
	5	Evaluation of Disinfectants
	6	Parts of Microscope
	7	Difference between Simple microscope & Compound microscope
	8	Difference between Light microscope & Electron microscope
	9	Difference between SEM and TEM
	10	Culture medium
	11	Guidelines for the Collection of Clinical Specimens
	12	Collection of Clinical specimens
	13	Transport of Clinical specimens
Week – 2	14	Stains and its types
	15	Simple staining
	16	Gram staining
	17	Acid fast staining
	18	Negative staining
	19	Endospore staining
	20	Metachromatic granule staining
	21	Flagella staining
	22	Hanging drop method
	23	LPCB staining
	24	KOH Wet mount
	25	Slide culture technique
	26	Germ tube test
Week – 3	27	Spread plate technique
	28	Pour plate technique
	29	Difference between Spread plate method and Pour plate method.
	30	Streak plate technique
	31	Catalase test
	32	Oxidase test
	33	Urease test
	34	Indole test
	35	Methyl red (MR) test
	36	Voges Proskauer (VP) test
	37	Citrate utilization test
Week – 4	38	Carbohydrates fermentation test
	39	Triple sugar iron (TSI) test
	40	Starch hydrolysis test
	41	Casein hydrolysis test
	42	Gelatin liquefication test
	43	Coagulase test
	44	Phenylalanine deaminase test
	45	Antibiotics sensitivity test

ONLINE COURSE SCHEDULE

WEEK	DATE	CONTENTS
Week - 1	07.05.2025	Week - 1 Pre-recorded Lecture Videos
Week – 2	14.05.2025	Week - 2 Pre-recorded Lecture Videos and Week – 1 Quiz
Week – 3	21.05.2025	Week - 3 Pre-recorded Lecture Videos and Week – 2 Quiz
Week – 4	28.05.2025	Week - 4 Pre-recorded Lecture Videos and Week – 3 Quiz
	04.06.2025	Week – 4 Quiz and Feedback form
	June 2025 Third Week	Distribution of Course Completion E-certificates (FDP/Internship/ International Online Certificate Course)

 Note

Links for Pre-recorded Lecture Videos will be shared to the Registered E.mail and WhatsApp Groups.