DIFFERENCE BETWEEN SPREAD PLATE METHOD AND POUR PLATE METHOD
Features |
Spread plate method |
Pour plate method |
Definition |
A technique used to count or isolate bacterial
colonies on the surface of the agar. |
A plate prepared by mixing the inoculum with the
cooled but still molten medium before pouring the latter into the Petri dish. |
Preparation |
Inoculum is spread on the solidified agar on a plate
by a spreader. |
Molten agar is poured on the inoculum in a Petri
dish and gently swirled. |
Amount of Inoculum |
0.1 ml |
1 ml |
Use of Glass L - Rod |
Uses a Glass L – Rod to spread
the sample evenly on the surface. |
Not used. |
Colony growth |
Only on the surface of the medium. |
In and on the medium. |
Area of Growth |
Less area to grow. |
More area to grow. |
Purpose |
To isolate specific clonal colonies. |
To count the number of colony forming bacteria in a
sample. |
Advantages |
Even distribution of colonies. |
Allows the identification of bacteria as Aerobes,
Anaerobes or Facultative aerobes. Allows the growth of Microaerophiles. |
Disadvantages |
Does not allow the growth of Microaerophiles. |
Picking a colony may interrupt other colonies. |
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